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Acabou de ser publicado o artigo com os dados do meu mestrado sobre o desenvolvimento de uma bolacha-do-mar na revista científica de acesso aberto PLoS ONE. O trabalho, escrito em inglês, é ilustrado com 16 pranchas (que incluem muitas das fotos daqui) e 10 cortes de vídeo com cenas do ciclo de vida deste equinodermo (mais ou menos as cenas inclusas neste vídeo); ainda tem uma larva em 3D de bônus. :D

Embryonic, Larval, and Juvenile Development of a Sea Biscuit

O artigo é livre para ser lido, comentado, baixado e re-usado de acordo com a licença de atribuição da Creative Commons (ver detalhes). Espero que gostem:

Embryonic, Larval, and Juvenile Development of the Sea Biscuit Clypeaster subdepressus (Echinodermata: Clypeasteroida)
Bruno C. Vellutini & Alvaro E. Migotto
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009654

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The website Virtual Urchin from Standford University has a series of interactive tutorials about different aspects of sea urchin biology. Including subjects are anatomy, predation and development. Last month they published an activity about the ocean acidification and its consequences for marine organisms with calcareous skeleton that included the vídeo A Sea Biscuit’s Life to exemplify the life cycle of echinoderms.

Life cycles of echinoderms at Virtual Urchin.

Life cycles of echinoderms at Virtual Urchin.

To know more visit virtualurchin.stanford.edu/AcidOcean.htm

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By the end of 2008 I received a message from the netlabel rec72 suggesting me to create a visual experience (videoclip) with images from A Sea Biscuit’s Life. The soundtrack would be a song fromthe album “The Scientific Method, Volume II Experiments in Sound Perspective” by Professor Kliq.

On the beginning of 2009 I began the editing. I used the same editor I ventured with “A Sea Biscuit’s Life”, Open Movie Editor, for linux. Since I never needed complex effects or transitions this program worked well (i.e., import, edit, insert graphics, basic transitions, and finally export without major constraints). However, to create a visual experience I decided to try additional effects (frei0r). I chose the track “Dream Sequence”, because I liked its ambience, and started to experiment.

Little by little the video began to appear, I disposed many scenes and selected final takes, until I finally decided to conclude. Since it is a visual experience there is no order, sometimes it gets a bit psychodelic, and so on… After some major technical problems with the effects (and the editor) the result was published on March 16 of 2009, and you can watch it below.

Dream Sequence / NeLaS & Professor Kliq from rec72 on Vimeo.

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The video A Sea Biscuit’s Life was played about 7000 times between November 17 and 18 and about 1500 visitors accessed the page yesterday (18/11). These numbers might not be expressive compared to Internet successes, but it is way beyond I was expecting!

I would like to thank everybody who left comments, sent messages and watched the video on the las 2 days! Specially those who helped with the promotion forwarding and writing about the video. I was really surprised with the repercussion and people’s enthusiasm, which made me quite happy.

I also would like to say that this video could not have been made without this great multimedia enthusiast of marine life, Alvaro Migotto.

And let this be only the beginning!

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It is my pleasure to announce that the video A Sea Biscuit’s Life is online! The video is about the life cycle of the sea biscuit Clypeaster subdepressus and includes footage of embryos, larvae and juveniles. It is part of my master’s thesis.

Watch the video!
mestrado.organelas.com/en/videos

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It was already time to speak out!

Well, this site was created to promote resources I’m producing during my graduate program (masther’s degree). Resources are mainly composed of photos and videos from the sea biscuit Clypeaster subdepressus from São Sebastião Channel (São Paulo, Brasil) during its development.

After 2 months of learning, installing, configuring, debugging, etc… I think this site is now ready to host these resources.

The site is organized in 2 parts. The gallery will host photos and videos and the blog where I’m going to put information about my project, evolution, tutorials, how to make videos, prepare photos, fertilize echinoderms…! It is also by the blog that you can interact with me leaving your comments on the posts. You can also contact me directly through the contact page.

Please send me suggestions for this site navigability or if you find any problems to visualize photos, videos or pages!

Resources will started to be uploaded on the second half of August for academic reasons.

For a start we have a video and an image!

This site content is free for academic uses. Please read carefully the terms of use and if you have any questions please contact me!

But who am I? Look here or browse through my personal website!

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Pluteus larva

Pluteus larva

Ventral view of a Clypeaster subdepressus larva under confocal microscopy. Material is a whole-mount of larvae ~36h after fertilization stained with carmine. After capturing a stack of images with a confocal microscope, the information of depth was codified to colors in a way that structures that have the same color are in the same focal plane. Afterwards, a projection combining allimages from the stack was created.

The stack was taken by A.S. de Souza from Instituto Butantan during a class from Toshie Kawano.

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