Ventral view of a Clypeaster subdepressus larva under confocal microscopy. Material is a whole-mount of larvae ~36h after fertilization stained with carmine. After capturing a stack of images with a confocal microscope, the information of depth was codified to colors in a way that structures that have the same color are in the same focal plane. Afterwards, a projection combining allimages from the stack was created.
The stack was taken by A.S. de Souza from Instituto Butantan during a class from Toshie Kawano.